OrangerV1, Main, Exploration, bibRecord, 003273

Agrobacterium-mediated transformation of citrange : factors affecting transformation and regeneration

Identifieur interne : 003273 ( Main/Exploration ); précédent : 003272; suivant : 003274

Agrobacterium-mediated transformation of citrange : factors affecting transformation and regeneration

Auteurs : M. Cervera [Espagne] ; J. A. Pina [Espagne] ; J. Juarez [Espagne] ; L. Navarro [Espagne] ; L. Pena [Espagne]

Source :

Plant cell reports [ 0721-7714 ] ; 1998.

RBID : Pascal:99-0031664

Descripteurs français

Pascal (Inist)
- Transformation génétique, Modalité traitement, Efficacité, Régénération, Milieu culture, Lumière, Composition chimique, Amélioration génétique, Agrobacterium tumefaciens, Auxine, Kanamycine, Citrus sinensis Poncirus trifoliata.

English descriptors

KwdEn :
- Agrobacterium tumefaciens, Application method, Auxin, Chemical composition, Culture medium, Efficiency, Genetic improvement, Genetic transformation, Kanamycin, Light, Regeneration.

Abstract

The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. Osbeck×Poncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during co-cultivation, cocultivation on a medium rich in auxins, post-cultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure.

Affiliations:

Espagne

Links toward previous steps (curation, corpus...)

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to stream PascalFrancis, to step Checkpoint: 000A87
to stream Main, to step Merge: 003519
to stream Main, to step Curation: 003273

Le document en format XML

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<term>Culture medium</term>
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<front><div type="abstract" xml:lang="en">The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. Osbeck×Poncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during co-cultivation, cocultivation on a medium rich in auxins, post-cultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure.</div>
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Agrobacterium-mediated transformation of citrange : factors affecting transformation and regeneration

Agrobacterium-mediated transformation of citrange : factors affecting transformation and regeneration

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri